BT-474 [BT474]人乳腺導(dǎo)管癌細(xì)胞
描述:E. Lasfargues 和 W.G. Coutinho從一個實心的乳腺浸潤性導(dǎo)管癌中分離到了BT-474細(xì)胞株。
動物種別:人
性別:女
組織來源:乳腺導(dǎo)管癌
形態(tài):上皮細(xì)胞
培養(yǎng)基和添加劑:DMEM培養(yǎng)基(GIBCO,貨號12800017,添加NaHCO3 1.5g/L),90%;胎牛血清,10%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE:Lasfargues EY , et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572 22660: Lasfargues EY , et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035 32275: Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997.