人結(jié)直腸腺癌細(xì)胞HCT-15
??【編號(hào)】:HCT-15 ??【名稱】:人結(jié)直腸腺癌細(xì)胞HCT-15 ??【規(guī)格】:株 ??【價(jià)格】:1800 ![]() HCT-15人結(jié)直腸腺癌細(xì)胞
描述:DNA fingerprinting證據(jù)表明,這株細(xì)胞和DLD-1 (ATCC CCL-221) 來源于同一個(gè)人;但同工酶及細(xì)胞染色體組型分析仍存疑問。 細(xì)胞呈CSAp陰性(CSAp-)。 角蛋白免疫過氧化物酶染色陽性。
動(dòng)物種別:人
性別:男
組織來源:結(jié)直腸腺癌
形態(tài):上皮細(xì)胞
培養(yǎng)基和添加劑:RPMI-1640(GIBCO,貨號(hào)31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;優(yōu)質(zhì)胎牛血清,10%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE:Schroy PC , et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 8625092 Journal of Interferon & Cytokine Research 24:219,2004. Brattain MG , et al. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 41: 1751-1756, 1981. PubMed: 7214343 Sun L , et al. Autocrine transforming growth factor-beta 1 and beta 2 expression is increased by cell crowding and quiescence in colon carcinoma cells. Exp. Cell Res. 214: 215-224, 1994. PubMed: 8082724 Santoro IM , Groden J . Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479 Brattain MG , et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756 Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064 Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486 Wang R , et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887 MOLECULAR THERAPY 11(4):531,2005www.atcc360.com
中文名稱:人結(jié)腸癌細(xì)胞
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