人正常前列腺上皮細胞RWPE-1
| ??【編號】：RWPE-1 ??【名稱】：人正常前列腺上皮細胞RWPE-1 ??【規格】：株 ??【價格】：   人正常前列腺上皮細胞
細胞名稱：RWPE-1人正常前列腺上皮細胞
描述：腫瘤抑制基因： p53 + [PubMed: 9214605] pRB + [PubMed: 9214605] 一位正常男性前列腺組織切片的周圍區域的上皮細胞用單拷貝的人乳頭瘤病毒的18(HPV-18)進行轉化，建立了RWPE-1 (ATCC CRL-11609) 細胞株 [PubMed: 9214605]. 在三維Matrigel培養時，在雄激素作用下，RWPE-1細胞形成腺胞并向培養基中分泌PSA。[PubMed: 11170142]. 當與Matrigel或基質細胞混合注射雄性裸鼠時，RWPE-1細胞也能形成腺胞[PubMed: 11304724] 并產生PSA。 來源于RWPE-1的細胞再用Kirstin鼠類腫瘤病毒轉染Ki-ras基因，建立了能成瘤的RWPE-2細胞株(ATCC CRL-11610) [PubMed: 9214605] 和 RWPE2-W99 (ATCC CRL-2853) 細胞株。 另外，用N-甲醇-N-硝基脲(MNU)處理RWPE-1，建立了一系列模擬前列腺癌進程中不同時期的成瘤細胞株。 它們是 WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850, WPE1-NB11 (ATCC CRL-2851) 和 WPE1-NB26 (ATCC CRL-2852) 細胞株。 據提供者報道，RWPE-1 細胞株(ATCC CRL-11609)經過檢測，乙肝、丙肝、人免疫缺陷病毒都呈陰性。
動物種別：人
性別：男
組織來源：器官： 前列腺 疾病： 正常 細胞類型： 上皮細胞
形態：上皮細胞
培養基和添加劑：這株細胞的基本培養基作為Invitrogen (GIBCO)提供的kit的部分： 角化細胞無血清培養基K-SFM，kit目錄號17005-042。隨kit提供這株細胞生長所需的兩種添加劑（牛垂體提取物BPE及人重組表皮生長因子EGF）。 配制完全生長培養基，需添加以下成分： 0.05 mg/ml BPE – 隨K-SFM提供 5 ng/ml EGF -隨K-SFM提供。 注意： 不要過濾完全培養基。 氣相： 空氣，95%；二氧化碳，5%。 溫度： 37.0°C。
供應限制：A。僅供研究之用。
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