白介素29(干擾素λ,人類,rDNA衍生)WHO參考試劑
??【編號】:NIBSC10/176 ??【名稱】:白介素29(干擾素λ,人類,rDNA衍生)WHO參考試劑 ??【規(guī)格】:個 ??【價格】:2600元 ![]() 白介素29(干擾素λ,人類,rDNA衍生)WHO參考試劑
編號:NIBSC10/176
英文:Interleukin-29 (Interferon Lambda, Human, rDNA derived) WHO Reference Reagent
標(biāo)準(zhǔn)類型: 非WHO參考資料
規(guī)格:1個
描述:1. INTENDED USE
This material is intended as a biological reference standard in bioassays
for IL-29 (Interferon Lamda 1).
2. CAUTION
This preparation is not for administration to humans or animals in
the human food chain.
The preparation contains human and bovine source material. The
preparation contains an excipient of human origin which has been
tested and found negative for HBsAg and HIV antibody. The
preparation has subsequently been tested and found negative for antiHCV
and HCV RNA by PCR. The bovine casein used as an excipient
was sourced from a country where bovine spongiform encephalopathy
(BSE) has not been found. As with all materials of biological origin,
this preparation should be regarded as potentially hazardous to health.
It should be used and discarded according to your own laboratory's
safety procedures. Such safety procedures should include the wearing
of protective gloves and avoiding the generation of aerosols. Care
should be exercised in opening ampoules or vials, to avoid cuts.
3. UNITAGE
The assigned potency of the preparation is 5,000 units of biological
activity per ampoule.
4. CONTENTS
Country of origin of biological material: United Kingdom.
Each ampoule contains the residue after freeze-drying of 1ml of
phosphate buffered saline that contained:
Approximately 500 nanograms of recombinant human IL-29
1.0% human serum albumin
0.3% bovine casein
The IL-29 protein was expessed in E.coli.
The ampoule does not contain bacteriostat.
5. STORAGE
For economy of use, it is recommended that the solution be sub-divided
into several small aliquots and stored at -40oC or below. Avoid repeated
thawing/freezing. Unopened ampoules should be stored at -20oC.
Please note: because of the inherent stability of lyophilized material,
NIBSC may ship these materials at ambient temperature.
6. DIRECTIONS FOR OPENING
DIN ampoules have an 'easy-open' coloured stress point, where the
narrow ampoule stem joins the wider ampoule body. Various types of
ampoule breaker are available commercially. To open the ampoule,
tap the ampoule gently to collect material at the bottom (labelled) end
and follow manufactures instructions provided with the ampoule
breaker.
7. USE OF MATERIAL
No attempt should be made to weigh out any portion of the freeze-dried
material prior to reconstitutionDissolve the total contents of the ampoule with 0.5ml of sterile distilled
water. Rinse the ampoule with 0.4ml of sterile distilled water and make up
the total volume to 1ml with sterile distilled water. This solution should
now contain IL-29 at a concentration of 5,000 U/ml. Use carrier protein
where extensive dilution is required. It is recommended that the IL-29
solution be used immediately or stored as indicated in section5.
8. STABILITY
Reference materials are held at NIBSC within assured, temperaturecontrolled
storage facilities and they should be stored on receipt as
indicated on the label. It is the policy of WHO not to assign an expiry date
to their international reference materials. Accelerated degradation studies
have indicated that this material is suitably stable, when stored at -20?C
or below, for the assigned values to remain valid until the material is
withdrawn or replaced. These studies have also shown that the material
is suitably stable for shipment at ambient temperature without any effect
on the assigned values. Users who have data supporting any
deterioration in the characteristics of any reference preparation are
encouraged to contact NIBSC.
9. REFERENCES
This reference reagent was produced under WHO guidelines cited in the
WHO Technical Reports Series, no. 925, 2005.
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